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Part:BBa_K1001752:Design

Designed by: Vansh Singh   Group: iGEM13_The_Agency_Escondido   (2013-05-02)


RFP and RBS behind Lamda Prm Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We specifically used the modified Lambda Prm Promoter because it was positively regulated.


Source

We use the intermediate part, BBa_S03873, formed from an RFP (BBa_E1010) and an RBS (BBa_B0034).

References